Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic AnalysisMegan Holt 1, Brandon Adams 1, Vidya Chandrasekaran 1
1Department of Biology, Saint Mary's College of California
This paper describes the isolation and culturing of embryonic rat sympathetic neurons from the superior cervical ganglia. It also provides detailed protocols for immunocytochemical staining and for preparing neuronal extracts for mass spectrometric analysis.
